La proteína nuclear neuronal (NeuN) es un marcador ampliamente utilizado para la investigación y el diagnóstico histopatológico de enfermedades del sistema nervioso. El desenlace fatal causado por la rabia puede atribuirse a cambios bioquímicos específicos que afectan gravemente la función neuronal. RESUMEN: La rabia es una enfermedad mortal causada por un virus neurotrópico que produce discretos cambios morfológicos difícilmente observables con la histopatología convencional. KEY WORDS: Rabies NeuN Cerebral cortex Mouse Immunohistochemistry Neurons. These results contribute to the knowledge of the dynamics of cellular infection on rabies pathogenesis. Either the immunoreactivity of NeuN or its expression is affected by the presence of rabies virus in the cerebral cortex depending on the inoculation route. In contrast, the changes were not statistically significant in mice inoculated intramuscularly. All parameters revealed decreased immunoreactivity for NeuN in cortical neurons of mice intracerebrally infected with rabies. Neuronal counts, cellular optical densitometry and neuronal diameter measurements were performed to analyze the immunoreactivity of the protein. Immunohistochemistry was used to study the expression of NeuN in the motor area of the cerebral cortex. The brains were removed and cut into coronal sections on a vibratome. Control mice were also euthanized at the same age. Rabies-infected mice were sacrificed at the terminal stage of the disease.
To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function.
SUMMARY: Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. * Grupo de Morfología Celular, Instituto Nacional de Salud (INS), Bogotá, Colombia. Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of MiceĮfecto Diferencial de la Vía de Inoculación del Virus de la Rabia sobre la Inmunorreactividad de NeuN en la Corteza Cerebral de RatonesĪura Caterine Rengifo* Vanessa Jazmin Umbarila* Mary Janeth Garzón* & Orlando Torres-Fernández*
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